Biophysical Properties of Intrinsically Disordered p130Cas Substrate Domain — Implication in Mechanosensing
Figure 6
Circular dichroism (CD) spectra of CasSD.
(A) CD spectrum of CasSD at 0.2 mg/mL concentration in 10 mM sodium phosphate pH 7.8 (black line). Standard spectra for reference samples with known secondary structures are also given for comparison. Thin orange: α-helix; Thin green: β-sheet; Red: polyproline II; Blue: random coil. (B) Increase of molar ellipticity in the 220–240 nm region of the CasSD CD spectrum with increasing concentration of urea.