Biophysical Properties of Intrinsically Disordered p130Cas Substrate Domain — Implication in Mechanosensing
Figure 5
Sedimentation velocity analytical ultracentrifugation of purified CasSD.
CasSD was subjected to analytical ultracentrifugation experiment in 10% (v/v) glycerol at a concentration of 1 mg/mL at 4°C over 7.3 hours. Data was fit to the continuous distribution model to obtain the experimental molecular weight, Stokes radius and frictional ratio of CasSD. (A) Raw data from the time-course measurement of absorbance of the sample at 280 nm along the sample cell length. (B) Residuals after fitting the data to the continuous size-distribution model. (C) Sedimentation coefficient distributions c(s) calculated from the sedimentation profile showing the calculated parameters in the inset.