Biophysical Properties of Intrinsically Disordered p130Cas Substrate Domain — Implication in Mechanosensing
Figure 3
Characterization of CasSD by analytical size-exclusion chromatography.
Purified CasSD was subjected to an analytical size-exclusion chromatography. Sample buffer and running buffer used were 10 mM potassium phosphate at pH 7.5, 100 mM potassium chloride, 1 mM EDTA and 5% (v/v) glycerol. Reference proteins were also subjected to the same chromatographic treatment for comparison. Red: ferritin; orange: catalase tetramer; green: bovine serum albumin; blue: ribonuclease A. Ferritin assumes a 440-kDa complex comprised of 24 subunits of light and heavy chains. Peaks eluting at a higher elution volume are thought to be ferritin molecules comprised of fewer subunits.