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Waste Not, Want Not: Why Rarefying Microbiome Data Is Inadmissible

Figure 1

A minimal example of the effect of rarefying on statistical power.

Hypothetical abundance data in its original (Top-Left) and rarefied (Top-Right) form, with corresponding formal test results for differentiation (Bottom).

Figure 1

doi: https://doi.org/10.1371/journal.pcbi.1003531.g001