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Assembly of Nsp1 Nucleoporins Provides Insight into Nuclear Pore Complex Gating

Figure 4

Height of Nsp1-FG chains.

(a) Time evolution of the average height. The height is shown for simulations for the end-tethered Nsp1-FGs in simulations wild-type_ring (green line), mutant_ring (red line), and random_array (cyan line). The heights are calculated as the average end-to-end distance in the -direction, the average being taken over all chains in a given simulation system. (b) Snapshot of the () end of simulation wild-type_ring (see also Figure 1(c)), mutant_ring, and random_array (see also Figure 3(c)). Videos show how during simulation wild-type_ring, the fully extended Nsp1-FG chains form brush-like, strongly cross-linked bundles (Video S1) and how in simulation random_array, the worm-like chain Nsp1-FGs form non-brush-like bundles (Video S3). The mutated Nsp1-FG chains (FG-to- AA) arising in simulation mutant_ring form brush-like bundles with similar average brush height as seen to arise for wild-type Nsp1-FGs in simulation wild-type_ring.

doi: https://doi.org/10.1371/journal.pcbi.1003488.g004