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Effect of Dedifferentiation on Time to Mutation Acquisition in Stem Cell-Driven Cancers

Figure 6

Fixation and exponential growth of two-mutation cells with dedifferentiation for variable stem cell population size.

A: Observed growth rate of the stem cell population (black curve) and the semi-analytic approximation Eq. (18) (green) for , , and . The vertical line denotes . B: Analytically predicted critical dedifferentiation rate as a function of asymmetric division probability and the growth advantage of the two-mutation progenitor population. Exponential growth occurs for . C: Normalized histogram (stars) of waiting times for exponential growth of the stem cell population with stochastic homeostasis and dedifferentiation for , . For comparison the histogram (red and black dots) as well as the analytical distributions of times to fixation given strict homeostasis for and are also shown. D: The median and inter-quantile range of times to first occurrence of two-mutation stem cells, given stochastic homeostasis and a range of dedifferentiation rates . For comparison, the waiting times to fixation for given strict homeostasis (shaded areas) for the equivalent value of are also shown. E: The probability that the first two-mutation stem cell arose from mutation in the stem cell compartment, rather than dedifferentiation. Vertical line denotes . Parameters for all simulations given in Table 1.

Figure 6

doi: https://doi.org/10.1371/journal.pcbi.1003481.g006