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In Silico Single-Molecule Manipulation of DNA with Rigid Body Dynamics

Figure 3

Comparison to force-extension curves.

Red circles: dimensionless stretching force as a function of the mean relative extension . Here the radius of the cylinders is corresponding to the mmol monovalent salt buffer used in [1]. The other parameters of the simulation are given in Table 1. For comparison, the black solid line reproduces the analytical Worm-Like-Chain force-extension approximation formula [35]. Black triangles correspond to a numerical fit of the exact Worm-Like-Chain model [34] with the same persistence length . Blue crosses: we also show simulations in the limit case , with no torsional rigidity () and no collisions, and compare it to the theoretical force-extension curve of a Freely-Jointed-Chain (FJC, blue solid line). The statistical error bars on the simulation points are all smaller than the symbol size.

Figure 3

doi: https://doi.org/10.1371/journal.pcbi.1003456.g003