PEX5 and Ubiquitin Dynamics on Mammalian Peroxisome Membranes
Figure 3
Uncoupled and directly coupled cargo translocation.
Both uncoupled and directly coupled translocation models have identical PEX5 and ubiquitination behavior and so they are reported together. (A) cytosolic PEX5-cargo concentration vs. cargo addition rate, . Different numbers of binding sites per importomer are shown from
(orange triangles) to
(green diamonds), as shown in the legend; the legend also applies to (B), (C), and (D). The dashed black line is the measured cytosolic PEX5 concentration of
[43]. This is consistent with
when
. (B) Peroxisomal PEX5 fraction vs.
. (C) Fraction of peroxisomal PEX5 that is ubiquitinated vs. PEX5 cargo addition rate,
. (D) Ubiquitin per peroxisome vs.
. A characteristic increase of ubiquitination with
is seen that is largely independent of the number of binding sites
. Vertical bars represent the standard deviation of observed values; error bars are smaller than point sizes.