Handling Uncertainty in Dynamic Models: The Pentose Phosphate Pathway in Trypanosoma brucei
Figure 6
(A) Effect of 6PGDH ablation on the growth rate. A non-induced 6PGDHRNAi culture, grown in glucose-containing HMI-9 was split at 0 h, +tet is induced with tetracycline, while −tet is the non-induced control. (B) Specific activities of 6PGDH in induced and control 6PGDHRNAi parasites. (C) Western blot showing predominant co-localization of 6PGDH with the glycosomal marker aldolase (fraction S), while a faint band can also be observed in the cytosolic fraction P with the marker enolase. (D) Cell densities during growth on different substrates. At t = 0 h, a 6PGDHRNAi culture grown on glucose was split at 1·105 ml−1 to HMI-9 with either glucose or fructose, and in the absence and presence of tetracycline. Plotted cell densities are cumulative, as −tet cultures were split at 48 h to 1·105 ml−1. A higher starting cell density was used to allow the parasites to adapt to the change in carbon source. Growth on fructose is slower than on glucose, but is unable to rescue the induced cultures.