Substrate-Driven Mapping of the Degradome by Comparison of Sequence Logos
Figure 6
Mapping of known targets of benzamidine to the substrate-driven protease specificity tree:
Known targets from the ChEMBL database (outer ring blue) cluster on top of the protease specificity tree based on S4-S1 substrate readout compared to unknown targets (outer ring light grey) and targets without ChEMBL identifier (outer ring white). Proteases are colored according to their catalytic type: serine proteases (cyan), metallo proteases (pink), cysteine proteases (dark grey) and aspartic proteases (blue). Targets of benzamidine are members of the chymotrypsin fold preferring positively charged amino acids at P1. Off-target binding of benzamidine to proteases positioned in vicinity of the already known targets (e.g. granzyme A) is proposed.