Cell-Based Multi-Parametric Model of Cleft Progression during Submandibular Salivary Gland Branching Morphogenesis
Figure 2
Cellular and cytoskeletal organization in developing salivary glands.
Epithelial cells express E-cadherin (red) during organ development and organize as polarized outer columnar cells (OCCs) and non-polarized inner polymorphic cells (IPCs) at (a) E12 and (b) retain this organization after 24 hours of growth. Cortical F-actin localization occurs during cleft formation at (c) E12 and (d) E12+24 hrs. Epithelial proliferation occurs in both outer and inner cell compartments shown with phospho-histone H3-labeled nuclei (red) relative to total nuclei with SYBR green (blue) at (e) E12 and (f) E12+24 hrs. Scale = 20 µm.