Dynamic Rendering of the Heterogeneous Cell Response to Anticancer Treatments
Figure 2
Experimental plan and first-level data analysis.
For FC analysis, cells from replicated samples were collected, fixed and stained at 6, 24, 48 and 72(A) Representative FC data: i) monoparametric cell cycle analysis, from which we calculated %G1, %S, %G2M, ii) biparametric DNA-BrdU analysis after BrdU pulse labeling, from which we calculated the percentages of BrdU-positive cells (%BrdU+), i.e. cells which were in S phase at the time of labelling, and undivided BrdU+ cells (%Und+). (B) Representative TL data: movies were collected and each cell was tracked with its descendants. At least 300 lineages were analysed in each treatment group. Three representative lineages are shown, where coloured squares indicate the outcome event of each cell. Analysis of lineage data gave population statistics like frequency distribution of intermitotic times and number of cells in each generation (bottom).