Reconstructing the Genomic Content of Microbiome Taxa through Shotgun Metagenomic Deconvolution
Figure 4
Reconstructing the genomic content of reference genomes from simulated mixed metagenomic samples using metagenomic deconvolution.
(A) ROC curves (AUC = 0.93) for predicting KO presence and absence across all species as a function of the threshold used to predict the presence of a KO. ROC curve for a naïve convolved prediction (AUC = 0.76) is illustrated for comparison. (B) Predicted genomic content of each species. KOs are partitioned into bins based on the set of genomes in which they are present (e.g., genes present only in the first species, genes present only in the second species, genes present in the first and second species but not in the third, etc.; see Venn diagram). The height of each bar represents the proportion of KOs in each bin and the color represents the presence of these KO in each species. The black strip inside each bar represents the fraction of KOs from this bin predicted to be present in each species.