Sensitive Detection of Viral Transcripts in Human Tumor Transcriptomes
Figure 6
Estimation of required cellular transcript abundances for achieving a given transcript coverage.
Sequencing coverage of viral transcripts is depending on the average number of transcript copies per cell in the sequenced sample, on the length of the viral transcript being sought, and on characteristics of the sequencing process. In order to better visualize the optimal sequencing depth required for detection of viral factors, we estimated the required number of transcript copies per cell for different sequencing depths. These sequencing depths are expressed as factors relative to the depths employed for the NB1/NB2 panel generated in this study (which are here reported as a relative sequencing depth of 1).