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Sensitive Detection of Viral Transcripts in Human Tumor Transcriptomes

Figure 4

Detection of low-coverage, homologous, and chimeric viral transcripts.

Displayed are performances of Virana, CaPSID, and RINS at detecting the three human-viral homologous gene regions Bo17, gag, and vABL. Performance is quantified in terms of sensitivity (right panel) and absolute number of reads correctly identified (left panel) at differing sequencing coverages ( fold). Methods are validated at detecting both isolated gene regions (upper part) as well as at detecting human-viral fusion transcripts involving each of the three gene regions fused to the human TP53 proto-oncogene (lower part). Specificity of detection is 1.0 (100%) for all detection methods (not displayed).

Figure 4

doi: https://doi.org/10.1371/journal.pcbi.1003228.g004