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Modeling the Effect of APC Truncation on Destruction Complex Function in Colorectal Cancer Cells

Figure 4

Comparison of simulated and observed effects of transfection of SW480 cells with APC constructs.

Relative levels in SW480 cells in response to transfection with the APC constructs of Fig. 3 are shown. The gray bars, which correspond to the left , represent experimental data from Munemitsu et al. [31]. The black bars, which correspond to the right , represent model predictions. The predicted concentrations (black bars) are each divided by the concentration of in a normal cell (35 nM, Table 1). For all APC constructs, the same transfection efficiency is assumed. We take a transfected cell to contain 100 nM of added protein. The predicted results therefore represent the effects of 100 nM of a construct in addition to 100 nM of endogeneous APC1338. The simulation results shown here were obtained using BioNetGen input files provided in the Supporting Information: Text S3 was used for the APC-B and APC-F cases, Text S4 was used for the APC-A case, Text S5 was used for the APC-C case, Text S6 was used for the APC-D case, and Text S7 was used for the APC-E case.

doi: https://doi.org/10.1371/journal.pcbi.1003217.g004