Modeling the Effect of APC Truncation on Destruction Complex Function in Colorectal Cancer Cells
Figure 3
Summary of APC constructs considered in simulated transfections and in the experimental study of Munemitsu et al. [31].
The 12 constructs used by Munemitsu et al. [31] are divided into six classes based on their structures. Proteins within the same class are functionally equivalent according to our model. A representative of Class A (APC-A) contains all three protein binding sites considered in the model for full-length APC. This class is regarded as equivalent to full-length APC. A representative of Class B (APC-B) contains 15-aa repeats and the first 20-aa repeat. This class is regarded as equivalent to APC1338, the truncated form of APC found in SW480 cells. A representative of Class C (APC-C) corresponds to a fragment that contains only the 15-aa repeats. A representative of Class D (APC-D) corresponds to a fragment that contains only the first 20-aa repeat. A representative of Class E (APC-E) corresponds to a fragment that contains the 20-aa and SAMP repeats. A representative of Class F (APC-F) corresponds to a nonfunctional fragment that contains none of the three APC sites included in the model.