How MicroRNA and Transcription Factor Co-regulatory Networks Affect Osteosarcoma Cell Proliferation
Figure 3
Functional implications of microRNA target genes in OS proliferation.
(A) Differentially expressed microRNA target genes. The heatmap shows the expression of DE microRNA target genes (log2 FC≥|0.7| & p-value<0.05, y-axis) among all OS cell samples (x-axis). High (dark blue) and low (light blue) proliferative OS samples are grouped into distinct clusters. The red/green color code corresponds to the expression deviation from the average expression among all samples. Complete-linkage clustering was applied with the Pearson correlation as distance metric. (B) Principal component analysis based on GO functional similarity. The figure draws the first (x-axis) and second (y-axis) principal components based on the GO functional similarity of microRNA target genes. The target genes are color coded according to their cluster membership determined by FCM. The radius of each gene represents the strength of association to its cluster. (C) Comparison of the GO enrichment analyses of the distinct clusters. Each row corresponds to a specific biological process category. The number of genes included in each category is indicated in the round brackets. Circle sizes correspond to the percentage of row-genes located in a cluster. Significantly enriched categories are color coded in red and random categories in blue.