Biochemical Competition Makes Fatty-Acid β-Oxidation Vulnerable to Substrate Overload
Figure 3
Steady-state fluxes and concentration of the intermediate metabolite at various concentrations of the substrate palmitoyl CoA.
Panels A and C: Steady-state flux through the FA β-oxidation. Panel B: CoASH concentration (solid lines) and total CoA esters (dashed lines) corresponding to the simulations in A. Panel D: Graphical representation of the differences between the computational models with and without competition. Blue lines in A and B: standard model; red lines in A and B: model without competition; green lines in A and B: model with fixed CoASH concentration (see text). Solid line in C: standard model (malonyl-CoA concentration 0 µM, mitochondrial NAD/NADH ratio 15); short-dashed line in C: standard model but with increased concentration of malonyl CoA of 10 µM; long-dashed line in C: standard model but with increased mitochondrial NAD+/NADH ratio of 40. Different colors in panel D represent the four acyl-CoA dehydrogenases; cyan: SCAD, yellow: MCAD, purple: LCAD and green: VLCAD.