Spatial Stochastic Dynamics Enable Robust Cell Polarization

doi:10.1371/journal.pcbi.1003139

Spatial Stochastic Dynamics Enable Robust Cell Polarization

Figure 4

Punctate Polarization (Green: Cdc42a, Red: Spa2).

(A) Yeast cells treated with -factor show the wider Cdc42a (marked by Ste20-GFP) and tighter Spa2 polarization. (B) Visualization of a stochastic realization of the polarisome model (white indicates regions with actin cables attached to the membrane). (C) Normalized fluorescence intensity membrane profiles of Ste20-GFP and Spa2-mCherry from a yeast cell undergoing polarisome formation (Green: Ste20 (Cdc42a). Dashed black: Ste20 fit. Red: Spa2). Ensemble mean experimental data is in Fig. S19. (D) Normalized membrane intensity profile from stochastic and deterministic realizations of polarisome model, the Cdc42a input, and the mean output of a stochastic ensemble (). Inset: Absolute membrane intensity profile from stochastic and deterministic realizations of polarisome model, and Spa2 ensemble mean. (Red: Spa2 Stochastic. Dashed blue: Spa2 deterministic. Black diamond: Spa2 ensemble mean. Green: Cdc42a (input)).

doi: https://doi.org/10.1371/journal.pcbi.1003139.g004