Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM
Figure 8
Supplementing with nutrients in alternate pathways can rescue some mutants.
The model makes the novel prediction that ace-2, ace-3, and ace-4 mutants (purple) in the TCA cycle can be rescued by supplementing minimal media with L-citrulline, L-arginine, L-ornithine, or L-glutamine (light blue) because each of these nutrients provide an alternate route via amino acid pathways to the essential metabolite 2-oxoglutarate (red).