Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM
Figure 7
Connection between glyoxylate cycle and gluconeogenesis reveals mechanistic insight into the nutrient rescue of acu mutants.
acu-3, acu-5 and acu-6 mutants are known to be lethal when acetate is the sole carbon source, because the glyoxylate cycle is blocked [88]. We correctly predict these mutants can be rescued by sucrose, and we additionally predict they can be rescued when supplemented with fructofuranose and glucose, because the enzymes encoded by acu-3, acu-5, and acu-6 are upstream of these sugars in the gluconeogenesis pathway.