Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM
Figure 6
Mechanistic insight into the nutrient rescue of cysteine and methionine metabolism.
The model correctly predicts that cys-5, cys-9, and cys-11 mutants can be rescued when the downstream nutrients sulfite and thiosulfate are provided in the media. Similarly, the model correctly predicts that met-2, met-5, met-6, met7 and met-8 mutants are rescued by L-methionine; met-2, met-5 and met-7 mutants are rescued by L-homocysteine; and met-5 and met-7 mutants are rescued by L-cystathione. The model makes the potentially novel predictions that hom-1 and all cys mutants can be rescued by the downstream supplements L-cystathione, L-homocysteine and L-methionine. The model also makes the potentially novel prediction that cys-4 is not rescued by either upstream nutrient supplements sulfite or thiosulfate.