Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM
Figure 2
(A) FBA does not require an input flux for cycles because it does not account for dilution of metabolites that participate in active reactions. (B) limed-FBA requires an input flux for cycles to compensate for dilution of metabolites that participate in active reactions. (C) FBA fails to correctly predict arg-14 gene essentiality because without an input flux, metabolite dilution prevents the isolated acetyl cycle compounds from being produced (side compounds not shown).