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Fibrin Networks Regulate Protein Transport during Thrombus Development

Figure 2

A: FRAP microscopy of fluorescently labeled Fab fragments of IgG.

For a typical FRAP experiment performed on a fibrin network, the regions of interest (ROIs, radius of 27 m) before, immediately after photobleaching, and after 98 s are shown (green circle). Normalized fluorescence recovery for the corresponding ROIs and their fits according to a model (Equation (7), ) are shown by symbols and a line, respectively. Fluorescence intensity values before () and after () photobleaching, and at the end of the experiment (I_∞), are shown. For each sample, FRAP measurements were repeated 3 times in 8 different locations over the sample and the arithmetic mean of the intensity curves was taken. B: Hydrodynamic diameter measurements by dynamic light scattering: circles denote -thrombin and squares denote Fab IgG.

doi: https://doi.org/10.1371/journal.pcbi.1003095.g002