Scrutinizing MHC-I Binding Peptides and Their Limits of Variation
Figure 5
(a) Distribution of prediction scores for sets of n SIINFEKL-fragment containing octapeptides. High scores indicate MHC-I H-2Kb binding predictions, while lower scores indicate non-binding predictions. Scores were computed with the final jury prediction model. (b) Binding of synthesized SIINFEKL-fragments to a MHC-I H-2Kb:IgG1 fusion protein relative to the average binding of the positive reference (SIINFEKL, 100%) and the unloaded fusion protein (NoLigand, 0%). Bars correspond to the arithmetic mean of quadruplicate measurements, with error whiskers depicting the volatility in terms of standard deviation.