Unsupervised Clustering of Subcellular Protein Expression Patterns in High-Throughput Microscopy Images Reveals Protein Complexes and Functional Relationships between Proteins
Figure 7
Subcellular location class profiles.
a) Time series for protein abundance in buds. Nuclear proteins are the last to appear in the bud (dashed line). b) The spatial distribution of protein expression is highly variable in the growing bud cell. Organelles appear to be pushed from the bud neck at the time of the nucleus inclusion (dashed line). Note that the absence of nuclear protein in the bud leads to irrelevant variations in the morphological distance features, perhaps due to auto-fluorescence captured in the GFP channel. Actin proteins migrate from bud tip to bud neck (black traces). c) In the mother cell, organelles appear to maintain a typical distance to the bud neck, expect for the nucleus.