Unsupervised Clustering of Subcellular Protein Expression Patterns in High-Throughput Microscopy Images Reveals Protein Complexes and Functional Relationships between Proteins
Figure 4
a) GFP intensity heatmap for several protein whose abundances are known to be cell-cycle dependent. b) Profiles for 3 proteins showing significantly higher expression level in large buds. ‘n’ is the number of mother-bud pairs used to infer each time series. 26 out of the 60 time points (indicated with markers) show coherent cell-stage specific deviation (permutation test, See Suppl. Figure S4). c) Examples of mother-bud pairs with the computed pixel size (pt) of the bud object (identical RFP/GFP intensity scale). The displayed cells were manually selected and then ordered by the computed bud size. Arrows indicate nuclear localization at lower intensity.