Unsupervised Clustering of Subcellular Protein Expression Patterns in High-Throughput Microscopy Images Reveals Protein Complexes and Functional Relationships between Proteins
Figure 1
Pipeline of the methods used in this work.
The identification of cells, assignment of cell type, cell stage and the estimation of cell confidence is based solely on the intensities of the RFP marker present in all strains. Please refer to the Results and Methods for descriptions of steps (i)–(vi). The cell type, stage and confidence are then used in conjunction with the GFP signal from tagged proteins in each strain in order to compute biologically interpretable features of protein expression.