Localization of Protein Aggregation in Escherichia coli Is Governed by Diffusion and Nucleoid Macromolecular Crowding Effect
Figure 2
Single-aggregate tracking analysis inside E. coli cells.
Coordinates along the x and y-axis are shown in red and black, respectively. Low frequency sampling trajectories (LF) are displayed using full lines and high frequency ones (HF) using open symbols. Light red and black swaths indicate + and −95% confidence intervals for the x- and y-axis data, respectively (for clarity, − and + intervals for the x- and y-axis data, respectively, are omitted) (A) Corrected mean displacement where uc(t) is the applied correction. For the y-component, the correction is the time-average of the y-coordinate. For the x-component, the applied correction is cell growth :
where L(t) is the cell half-length at time t and Δt is the time interval between two consecutive images. (B) Corresponding mean squared displacements
. The inset shows a magnification of the HF results and their close-to-linear behavior for the first 10–15 seconds (dashed line).