Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
Figure 7
Comparison of predicted versus empirical distributions of flow-values for homopolymers of length 1–5.
Predicted (solid line) and empirical distributions (dotted line) of flow-values for homopolymers of length 1–5 (colours - black, red, green, blue and teal), for flow cycles 2,5,9 (rows) and PIC 1,10 and 12 (columns) for species B. amyloliquefaciens. The low number of observations of homopolymers of length 5 is the likely cause for abnormal distributions for this homopolymer length. The ‘shoulders’ observed in the data are often due to unexpectedly high popularity of boundary flow-values (eg. 0.51, 1.49, 1.51…).