Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
Figure 6
The influence of position in cycle (PIC, labelled 0–31) on flow-value distributions and consequently error rate and type.
Panel (a) shows the coefficient (main-effect) of each flow cycle position as predictors of mean of the flow-value distribution. Panel (b) shows the error rate broken down by insertions and deletions for each PIC. These do not include flow-values for homopolymers where the reference homopolymer length is zero.