Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
Figure 4
Examples of over-call/under-call errors in homopolymers of length less than 2.
By aligning the read (derived from the rounded flow-values), and its corresponding reference sequence (considered the ‘true’ sequence) at the flow level, we can identify examples of over-calling a zero-length homopolymer (Flow Cycle #2), and under-calling a one-length homopolymer (Flow Cycle # 6). Flow Cycle #5 demonstrates a zero-length homopolymer being correctly called as zero.