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Lifespan Differences in Hematopoietic Stem Cells are Due to Imperfect Repair and Unstable Mean-Reversion

Figure 8

Breakdown of Mean-Reverting Behavior in the Failure Rate Kinetics of Long-term Repopulating HSCs.

A: An experimental failure rate kinetic (blue scatter-line plot; values vertical axis) compared to the kinetics of 100 realizations (thin red lines) of an Ornstein-Uhlenbeck process over the lifespan period (horizontal axis) of a clone with lifespan months. The realizations of the process were obtained using the iteration schema in eq 4. The same values of , and as in the experimental data were used. For simplicity, the initial condition was set at for (equivalent to assuming a load-free transplant). The important observation is that without additional conditions on the Ornstein-Uhlenbeck process, the expected behavior of the kinetic generated from data (blue curve) will not occur. B: The moving average (vertical axis; window size = 6) of the same failure rate kinetic as in Part A (blue line-scatter curve) reveals that the parameter increases slowly during the mean-reverting regime (raw moving average data (denoted “Moving Avg ”) are in black). The slow increase changes to rapidly increasing failure rates at around 82% of the lifespan. Both behaviors combine into the model of equation 9 with parameters , and (p-values = , , , respectively; ).

Figure 8

doi: https://doi.org/10.1371/journal.pcbi.1003006.g008