Lifespan Differences in Hematopoietic Stem Cells are Due to Imperfect Repair and Unstable Mean-Reversion
Figure 3
Reliability and Failure Kinetics of a Long-lived Long-term Repopulating HSC.
A–D: Four types of kinetics were calculated (compare Algorithm 1) from experimental kinetics for all clonal cell populations together (black), and the myeloid (green), T lymphocyte (red), and B lymphocyte (gray) cell populations, separately. In the representative example shown, notation is as in Algorithm 1 (applied to a single kinetic, i.e. batch size ). Also shown are the respective kinetics for the population of clonal hematopoietic stem cells (HSCs; blue). Since population data are difficult to obtain for stem cells directly, the HSC-related kinetics were inferred from the other data. This was accomplished by first predicting the reliability (Part B, blue curve) using the structure balance eq 1 and, then, deriving the other kinetics (blue curves for C, D, then A) with the methods of Algorithm 1.