< Back to Article

Connecting the Kinetics and Energy Landscape of tRNA Translocation on the Ribosome

Figure 5

Rates, free-energy barriers and crossing attempt frequencies.

Using , , and , the rates of barrier crossing were calculated as functions of the barrier heights for A) body rotation, B) head swivel, and C) tRNA displacements. From the rates, the barrier-crossing attempt frequencies , , and were derived (D–F). With these values, the energy landscape may be quantified for any kinetic scheme that can be decomposed into body rotation, head swivel and tRNA displacement. Dashed lines mark 5 and (range of rates measured for translocation [59]–[64]), providing an upper-limit range for the barrier height associated with each substep of translocation.

doi: https://doi.org/10.1371/journal.pcbi.1003003.g005