Connecting the Kinetics and Energy Landscape of tRNA Translocation on the Ribosome
Figure 2
Explicit-solvent simulation of an E. coli ribosome (water molecules and ions not shown), colored by region: 23S/5S rRNA (gray), 16S rRNA (cyan), proteins (light blue), P/P tRNA (red) and A/A tRNA (yellow). During translocation, tRNA molecules adopt hybrid configurations (middle). Rotation of the 30S body () and head (
) is associated with tRNA movement between binding sites. Here, we initiated the simulation in the pre-translocation configuration and characterized the structural fluctuations about the classical tRNA configuration and unrotated subunit configuration.