Differential Expression Analysis for Pathways
Figure 9
Simulated data on the post-transcriptional silencing by small RNAs, power vs. pathway effect, variance, and sample size.
At the top, the Reactome post-transcriptional silencing by small RNAs pathway is illustrated, with green, red, and grey nodes representing nodes whose simulated values were +μ, −μ, and 0, respectively [14]. The nodes are colored to indicate activity leading to silencing by cleaved RNA with 5′ phosphate and 3′ hydroxyl. At the bottom, power for detecting significant differential expression in this pathway is illustrated with respect to pathway effect, variance, and sample size.