Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
Figure 7
Expression of embryonic and adult stem cell genes in ES, iPS, FS, and blastemas.
All samples are ratioed to the time zero axolotl juvenile time course control. While ES and iPS cells show upregulation of a variety of stem cell genes including the key ES transcription factors POU5F1 and NANOG, the axolotl blastemas do not show upregulation of POU5F1, SOX2, and NANOG, but do show upregulation of adult stem cell markers (such as SALL4 and HMGA2). Note the burst of oncogenes (KLF4, MYC) early in the time course and the upregulation of adult stem cell genes later in the time course.