Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
Figure 2
Expression patterns of RNA-seq and qPCR along the time course.
Plots of the log2 ratios of TPMs (transcripts per million) of each time point relative to the time zero control for RNA-seq data (blue), and the log2 ratios of the qPCR expression measure (red) relative to the zero hour control. The R2 Pearson correlation across the time course is shown for each gene as well. The expression patterns are similar between the RNA-seq and qPCR data (average Pearson correlation for the four genes shown = 0.839, average Pearson correlation for all 19 genes = 0.741). See Figure S2 for the 15 additional genes not shown in Figure 2, and Table S2 for all Log2 ratios of RNA-seq and qPCR data. See Materials and Methods for an explanation of determining the ratios and for the Pearson calculation.