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Positively Selected Sites in Cetacean Myoglobins Contribute to Protein Stability

Figure 5

Divergence of cetaceans and the increase in Mb concentration by ∼10–20 fold.

The experimental folding stability of apoMb is added to the difference in stability of holo and apoMb reported for horse heart Mb (2.7 kcal/mol). Stability is highly correlated with Mb concentration with correlation coefficient ρ = 0.88 and p-value = 0.000331. The Mb concentration has been measured in adorsi and in bpsaos muscle types. Data are taken from 1: [57], 2: [75], 3: [44], 4: [7] and 5: [76]. All the folding stabilities are taken from [14].

Figure 5

doi: https://doi.org/10.1371/journal.pcbi.1002929.g005