Interconnected Cavernous Structure of Bacterial Fruiting Bodies
Figure 6
Detailed analysis of OCT scan.
Left Column Panel: A 2D rendered cross section from sequence of OCT slices. A) Raw data cross section. B) Gray-Scale image used for image analysis. The elliptical domains for radial density are overlayed on the image. C) Zoomed in region of (B). D) Analysis shows the average intensity (black line) and intensity distribution for cross-sectional discs as a function of height from the base of the mound. The x-axis is the height of each disc in the mound where zero is the base. Data is from mound shown in figure 5. The left plot is the unnormalized data that shows how many pixels have a given intensity value. Middle plot shows the distribution within each disc normalized by the area (total pixels) of each disc. Right plot shows the results from smoothing the distribution of each disc. The black rectangle in all three plots corresponds to the distribution for the cross-sectional disc in (A). E) Analysis showing radial density for four of the elliptical discs at different heights within the mound. Data is from the fruiting body shown in figure 5. The line is from cross-section shown in (B).