Calcium Wave Propagation in Networks of Endothelial Cells: Model-based Theoretical and Experimental Study
Figure 6
Experiment image and normalized intensity of cells in single fine line subjected to double stimulus.
(A) Image of a finite single fine line of cells subjected to dual mechano-stimulation. The stimulating probes are visible at the top-left and bottom-right of the image. The response time of cells labeled in red was calculated from the maximum rate of change of the fluorescence intensity with uncertainties 0.6 s. The response time of cells labeled in green was calculated from a running average of the fluorescence intensity with uncertainties 1.2 s. The first response time for cell 4 (1), represents the time of the first sharp rise in fluorescence versus time. The second response time of cell 4 (2), indicates the time when the fluorescence intensity increases a second time. Cells 4 and 5 represent the region where the calcium pulses are anticipated to meet. (B) and (C) present the normalized intensity of fluorescence of individual cells as a function of time.