PERT: A Method for Expression Deconvolution of Human Blood Samples from Varied Microenvironmental and Developmental Conditions
Figure 2
NNML recovers known compositions of immune cell line mixtures.
Microarray data of IM-9 (○), Jurkat (▵), Raji (□), THP-1 (+), and the mixtures of these four cell lines in known proportions were obtained from Abbas et al. (2009). Proportions of each cell line were predicted using (A) NNLS with cell line signature probes (reproduced from Abbas et al. (2009)), (B) NNLS without cell line signature probe, (C) NNML with cell line signature probes, and (D) NNLS without cell line signature probes. Model predictions were compared with the input proportions used to create the mixtures. Cell line signature probes were obtained from Abbas et al. (2009).