3D Reconstruction and Standardization of the Rat Vibrissal Cortex for Precise Registration of Single Neuron Morphology
Figure 5
Cortical curvature leads to anisotropic overlap of barrel columns.
(A) The granular layer is subdivided into barrels and the septum between barrels. The vectors describe the direction along the row (e.g., D1-D2-D3), arc (e.g., C2-D2-E2) and the 3-2-1 direction (e.g., C3-D2-E1). (B) Top: overlap of neighboring barrel columns in one reconstructed barrel cortex in different directions, based on a cylindrical extrapolation of the column. The magnitude of the overlap is influenced by the distance between neighboring columns and the magnitude of the curvature in different directions. Bottom: average values across all columns and all reconstructions. Error bars are 1 standard deviation. (C) Measurement of the average volume inside barrel columns and septa in all reconstructed barrel cortices. Error bars are ±1 standard deviation.