3D Reconstruction and Standardization of the Rat Vibrissal Cortex for Precise Registration of Single Neuron Morphology
Figure 4
3D reconstruction of barrels and barrel columns.
(A) Segmented barrel contours are smoothed in the z-direction to remove segmentation artifacts. (B) Anatomical structures are reconstructed in 3D. Blood vessels (orange) are reconstructed as 3D lines. Pia and WM are reconstructed as surfaces. The BC axis (dashed line) is found based on directions of blood vessels located around the BC and the orientation with respect to the pia. The column orientation is computed with respect to the C2 column. (C–E) Average dimensions of barrels and barrel columns are arranged on a grid in the layout of the barrel field. The arrows indicate the direction of the average gradient of the parameters.