Incorporating 16S Gene Copy Number Information Improves Estimates of Microbial Diversity and Abundance
Figure 6
The strength of correlations between true abundance (ni) versus observed gene abundance (gi) or estimated relative abundance () for 100 simulated communities generated by drawing 100 taxa from the 484-taxon reference phylogeny followed by estimation of the phylogenetic placement and copy number for those taxa.
We simulated phylogenetic placement and copy number estimation using full-length 16S sequences and sequences trimmed to the 351 bp V2V3 hypervariable region to simulate pyrosequencing data. Letter codes at top of panel indicate simulations that differed according to a Tukey HSD test (P<0.05; simulations that share a letter not significantly different).