Confidence-based Somatic Mutation Evaluation and Prioritization
Figure 6
Comparison of different experimental settings and analysis procedures.
A Estimated ROC curves for the comparison of the three different software tools (duplicates, 38× coverage). B Estimated ROC curves for the comparison of different average sequencing depths (SAMtools, no replication). 38× denotes the coverage obtained by the experiment, while other coverages were down sampled starting with this data. C Estimated ROC curves visualizing the effect of experiment replication (38× coverage, SAMtools). D Estimated ROC curves for different sequencing protocols (SAMtools, no replication). The curves were calculated using the results of the 2×100 nt library (Note: A complete display of the results can be found in Supplementary Figures S2 and S3 in Text S1. Also, unscaled versions of the plots are shown in Supplementary Figure S8 in Text S1, giving an impression of the individual set sizes).