Sub-diffraction Limit Localization of Proteins in Volumetric Space Using Bayesian Restoration of Fluorescence Images from Ultrathin Specimens
Figure 8
The effect of noise on the spatial frequency recovery of RL deconvolution.
(A) This graph clearly illustrates the effect of Poisson noise on the fidelity of RL deconvolution. Poisson noise was artificially generated and added to the same image stack, and the RL was performed on those images, then the MTF of the images were calculated to demonstrate that even with a 5% injection of noise the spatial frequency recovery was significantly degraded, and for a 10% noise increase for most frequencies of the MTF the ATD is not better than ATW. Pixel size of the images were 100 nm.