Sub-diffraction Limit Localization of Proteins in Volumetric Space Using Bayesian Restoration of Fluorescence Images from Ultrathin Specimens
Figure 5
Comparison of microtubules imaged using SIM versus deconvolution.
(A) Max projection of identical tissue volumes (ten 70 nm sections) imaged in SIM, ATW and ATD. Scale Bar = 10 um. (B–C) We quantify two parallel microtubules at the cross sections marked in the blow-up images in (B). Intensity cross sections (C) along the length of the microtubules show that the peaks of the microtubules are clearly resolved in the deconvolved and SIM scenario, whereas the peaks in the wide-field images are barely separated. The intensity of deconvolved image is higher, because it represents the computationally returned light at that pixel from the more blurred original image. For a more quantitative measurement of the similarity between SIM, ATD and ATW images, the Pearson's correlation coefficient is calculated using the images, which is: SIM to ATW = 0.8394. SIM to ATD = 0.8862, and ATW to ATD = 0.8982. The numbers confirm that in reference to the SIM, ATD is a closer match than ATW.